Journal: PLoS ONE

Article Title: Pre-eclampsia is associated with altered expression of the renal sodium transporters NKCC2, NCC and ENaC in urinary extracellular vesicles

doi: 10.1371/journal.pone.0204514

Figure Lengend Snippet: Antibodies used in the study.

Article Snippet: Anti-NKCC2 , Rabbit polyclonal , Rat N-terminus aa 33–55 , Stress-Marq.

Techniques:

Journal: PLoS ONE

Article Title: Pre-eclampsia is associated with altered expression of the renal sodium transporters NKCC2, NCC and ENaC in urinary extracellular vesicles

doi: 10.1371/journal.pone.0204514

Figure Lengend Snippet: (A) Representative Western blot from normal controls (NC), women with normal pregnancies (NP) and with pre-eclampsia (PE) probed with antibodies against NKCC2, pNKCC2 (S130 and T101/105), and CD9. (B) Densitometric analysis of total NKCC2 normalised for CD9 in the three groups. Difference between groups by Kruskall-Wallis test p<0.002. (C and D) Densitometric analysis of total NKCC2 pS130 normalised for total NKCC2 (C) and CD9 (D) in the three groups. Difference between groups by Kruskall-Wallis test p<0.0001 for both comparisons. (E and F) Densitometric analysis of total NKCC2 pS130 normalised for total NKCC2 (E) and CD9 (F) in the three groups. Difference between groups by Kruskall-Wallis test p<0.01 for (E) and not significant for (F). Individual comparisons by Dunns’ test *p<0.05, **p<0.01, ****p<0.0001.

Article Snippet: Anti-NKCC2 , Rabbit polyclonal , Rat N-terminus aa 33–55 , Stress-Marq.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Pre-eclampsia is associated with altered expression of the renal sodium transporters NKCC2, NCC and ENaC in urinary extracellular vesicles

doi: 10.1371/journal.pone.0204514

Figure Lengend Snippet: Detection of bands for phosphorylated forms of NKCC2 and NCC, and for ENaC proteins.

Article Snippet: Anti-NKCC2 , Rabbit polyclonal , Rat N-terminus aa 33–55 , Stress-Marq.

Techniques:

Journal: PLoS ONE

Article Title: Pre-eclampsia is associated with altered expression of the renal sodium transporters NKCC2, NCC and ENaC in urinary extracellular vesicles

doi: 10.1371/journal.pone.0204514

Figure Lengend Snippet: (A) NKCC2 pS130 vs urine protein/creatinine ratio, p = 0.014, n = 19. (B) γ-ENaC 50kD species vs urine protein/creatinine ratio, p = 0.001, n = 19. (C) γ-ENaC 50kD species vs urine protein/creatinine ratio, p = 0.002, n = 19.

Article Snippet: Anti-NKCC2 , Rabbit polyclonal , Rat N-terminus aa 33–55 , Stress-Marq.

Techniques:

Journal: PLoS ONE

Article Title: Pre-eclampsia is associated with altered expression of the renal sodium transporters NKCC2, NCC and ENaC in urinary extracellular vesicles

doi: 10.1371/journal.pone.0204514

Figure Lengend Snippet: Linear regression analysis of sodium transporter densitometry as a ratio with CD9.

Article Snippet: Anti-NKCC2 , Rabbit polyclonal , Rat N-terminus aa 33–55 , Stress-Marq.

Techniques:

Journal: Cell reports

Article Title: Magnesium and Calcium Homeostasis Depend on KCTD1 Function in the Distal Nephron

doi: 10.1016/j.celrep.2020.108616

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: rabbit anti-NKCC2 , Cell Signaling Technologies , Cat# 38436, RRID:AB_2799134.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Plasmid Preparation

Journal: Kidney international

Article Title: Renal tubular dysfunction in patients with American cutaneous leishmaniasis.

doi: 10.1038/ki.2011.251

Figure Lengend Snippet: Figure 2 | Western blotting analysis in the urinary exosome fraction, normalized to urinary creatinine, in controls and in patients with ACL. Abundance of (a) AQP2 and (b) NKCC2. ACL, American cutaneous leishmaniasis; AQP2, aquaporin 2; NKCC2, Na-K-2Cl cotransporter.

Article Snippet: We obtained the peptide-derived polyclonal antibodies specific to AQP2, NKCC2, NHE3, and pendrin from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Western Blot